Diazotrophic microorganisms are taxonomically and metabolically diverse and they populate a wide variety of habitats. Diazotroph diversity is typically quantified through the analysis of nitrogenase nifH gene sequences recovered from environmental …
The nifH gene is the most widely sequenced marker gene used to identify nitrogen-fixing Bacteria and Archaea. Numerous PCR primers have been designed to amplify nifH, but a comprehensive evaluation of nifH PCR primers has not been performed. We …