amplicon sequence analysis

Effects of H2 : CO2 ratio and H2 supply fluctuation on methane content and microbial community composition during in-situ biological biogas upgrading

Background: Commercial biogas upgrading facilities are expensive and consume energy. Biological biogas upgrading may serve as a low-cost approach because it can be easily integrated with existing facilities at biogas plants. The microbial communities …

Diazotroph community characterization via a highthroughput nifH amplicon sequencing and analysis pipeline

The dinitrogenase reductase gene (nifH) is the most widely established molecular marker for the study of nitrogen-fixing prokaryotes in nature. A large number of PCR primer sets have been developed for nifH amplification, and the effective deployment …

Microbial community characterization during anaerobic digestion of Scenedesmus spp. under mesophilic and thermophilic conditions

Microbial communities were thoroughly characterized in a mesophilic anaerobic membrane bioreactor (AnMBR) and a thermophilic continuous stirred tank reactor (CSTR), which were both treating recalcitrant microalgal biomass dominated by Scenedesmus. …

The effect of temperature and retention time on methane production and microbial community composition in staged anaerobic digesters fed with food waste